The production and recovery analysis of a promising recombinant phytase from Yersinia intermedia expressed in Escherichia coli and its potential use in biotechnology

Authors

  • William James Nogueira Lima Universidade Estadual de Montes Claros, Postgraduate Program in Biotechnology, Bioprocess Development Laboratory, Montes Claros, MG, Brazil. e Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0000-0002-1128-1448
  • Ruth Maria Rocha Ribeiro Universidade Estadual de Montes Claros, Postgraduate Program in Biotechnology, Bioprocess Development Laboratory, Montes Claros, MG, Brazil https://orcid.org/0009-0002-9728-5366
  • Sandro Braga Soares Universidade Estadual de Montes Claros, Postgraduate Program in Biotechnology, Bioprocess Development Laboratory, Montes Claros, MG, Brazil. https://orcid.org/0000-0002-5342-7230
  • Quemuel Quesley Nunes Mendes Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0009-0008-8521-9218
  • Sr. Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0009-0005-1777-7339
  • Amanda Ferrari das Neves Neves Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0009-0002-3428-5246
  • Nathália Zenaide Durães Soares Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil https://orcid.org/0000-0001-8083-5195
  • Ludmila Ribeiro Mendes Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0009-0007-7146-9075
  • Izabella Mendes Lima Universidade Estadual de Montes Claros, Postgraduate Program in Biotechnology, Bioprocess Development Laboratory, Montes Claros, MG, Brazil. https://orcid.org/0009-0007-9465-1195
  • Ivan Pires de Oliveira Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil https://orcid.org/0000-0003-1020-4376
  • Gustavo Leal Teixeira Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0000-0001-7293-0790
  • Bruna Mara Aparecida de Carvalho Mesquita Universidade Estadual de Montes Claros, Postgraduate Program in Biotechnology, Bioprocess Development Laboratory, Montes Claros, MG, Brazil. e Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0000-0001-8980-8599
  • Gabriela da Rocha Lemos Mendes Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0000-0001-6432-0239
  • Igor Viana Brandi Universidade Estadual de Montes Claros, Postgraduate Program in Biotechnology, Bioprocess Development Laboratory, Montes Claros, MG, Brazil. e Universidade Federal de Minas Gerais, Institute of Agricultural Sciences, Montes Claros, MG, Brazil. https://orcid.org/0000-0001-6714-7996
  • Mariana Sousa Vieira Universidade Federal de São João Del-Rei, Laboratory of Microorganism Biotechnology, Divinópolis, MG, Brazil. e National Institute of Science and Technology in Industrial Biotechnology, Brasília, DF, Brazil. https://orcid.org/0000-0002-2764-0863
  • Renato Ramos Godoi Universidade Federal de São João Del-Rei, Laboratory of Microorganism Biotechnology, Divinópolis, MG, Brazil. e National Institute of Science and Technology in Industrial Biotechnology, Brasília, DF, Brazil. https://orcid.org/0009-0004-4430-0897
  • Thais Paiva de Porto Souza Universidade Federal de São João Del-Rei, Laboratory of Microorganism Biotechnology, Divinópolis, MG, Brazil. e National Institute of Science and Technology in Industrial Biotechnology, Brasília, DF, Brazil. https://orcid.org/0009-0001-3681-5733
  • Marina Quadrio Raposo Branco Rodrigues Universidade Federal de São João Del-Rei, Department of Biotechnology, São João del-Rei, MG, Brazil e National Institute of Science and Technology in Industrial Biotechnology, Brasília, DF, Brazil. https://orcid.org/0000-0003-0302-5740
  • Daniel Bonoto Gonçalves Universidade Federal de São João Del-Rei, Department of Biotechnology, São João del-Rei, MG, Brazil e National Institute of Science and Technology in Industrial Biotechnology, Brasília, DF, Brazil. https://orcid.org/0000-0002-8178-1026
  • Alexsandro Sobreira Galdino Universidade Federal de São João Del-Rei, Laboratory of Microorganism Biotechnology, Divinópolis, MG, Brazil. e National Institute of Science and Technology in Industrial Biotechnology, Brasília, DF, Brazil. https://orcid.org/0000-0002-8890-3030

DOI:

https://doi.org/10.5327/fst.00433

Keywords:

downstream process, fermentation, animal nutrition, enzymes, cell disruption

Abstract

Recombinant phytases produced in Escherichia coli offer an alternative to inorganic phosphorus (P) use in monogastric diets, reducing production costs and minimizing soil and water pollution. The enhanced bioavailability of phytic P, minerals, proteins, and carbohydrates, along with the benefits of inositol, is highly valued by specialists when formulating feeds with optimal levels of enzymatic additives.

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Published

2025-04-30

How to Cite

Lima, W. J. N., Ribeiro, R. M. R., Soares , S. B., Mendes , Q. Q. N., Soares, J. P., Neves, A. F. das N., Soares , N. Z. D., Mendes , L. R., Lima , I. M., Oliveira , I. P. de, Teixeira, G. L., Mesquita, B. M. A. de C., Mendes , G. da R. L., Brandi , I. V., Vieira , M. S., Godoi , R. R., Souza , T. P. de P., Rodrigues , M. Q. R. B., Gonçalves, D. B., & Galdino , A. S. (2025). The production and recovery analysis of a promising recombinant phytase from Yersinia intermedia expressed in Escherichia coli and its potential use in biotechnology. Food Science and Technology, 45. https://doi.org/10.5327/fst.00433

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