Listeria monocytogenes, Coxiella burnetii, and Bacillus cereus in fresh cheese illegally marketed without inspection

Authors

  • Ana Carolina de SÁ Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0003-2356-2121
  • Leonardo Ereno TADIELO Universidade do Estado de Santa Catarina, Department of Animal Production and Food, Lages, SC, Brazil. https://orcid.org/0000-0003-4731-849X
  • Emanoelli Aparecida Rodrigues dos SANTOS Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0001-6011-3232
  • Camila Koutsodontis CERQUEIRA-CÉZAR Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0003-1222-6544
  • Layara Chuvukian CHINAQUE Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0002-4064-2118
  • Lára Cristina Bastos JULIANO Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0003-0863-3972
  • Janaina Prieto de OLIVEIRA Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0002-6031-1527
  • Evelyn Cristine da SILVA Universidade Estadual Paulista “Júlio de Mesquita Filho”, Institute for Biotechnology, Botucatu, SP, Brazil. https://orcid.org/0000-0003-0722-7710
  • Ana Paula FLAMÍNIO Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0002-7782-2163
  • Otávio Augusto MARTINS Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0003-0926-9690
  • Mateus de Souza Ribeiro MIONI Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculty of Agricultural and Veterinary Sciences of Jaboticabal, Jaboticabal, SP, Brazil. https://orcid.org/0000-0001-7886-1570
  • Antonio Carlos PAES Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0001-7774-927X
  • Fabio Sossai POSSEBON Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0002-0118-6164
  • Juliano Gonçalves PEREIRA Universidade Estadual Paulista “Júlio de Mesquita Filho”, School of Veterinary Medicine and Animal Science, Botucatu, SP, Brazil. https://orcid.org/0000-0002-8713-7506

DOI:

https://doi.org/10.5327/fst.000381%20

Keywords:

dairy products, foodborne diseases, milk, microbiology, polymerase chain reaction

Abstract

This study aimed to assess hygienic-sanitary indicators and the presence of pathogenic agents in fresh cheeses produced through informal methods. A total of 51 samples of fresh cheese, which were produced without sanitary inspection and sold informally, were analyzed for the presence of Listeria monocytogenes, Salmonella spp., Bacillus cereus, coagulase-positive Staphylococcus, Escherichia coli, Pseudomonas spp., Mycobacterium bovis, Mycobacterium tuberculosis, and Coxiella burnetii using microbiological and biomolecular analyses. The results revealed the detection of Listeria monocytogenes serotype 4b in one sample (1.96%), Bacillus cereus in two samples (3.92%), and Coxiella burnetii in four samples (7.84%). Furthermore, 28 samples (54.9%) exceeded the allowable limit for Escherichia coli counts as per legislation, and 23 samples (45.09%) had coagulase-positive Staphylococcus counts above the microbiological limit specified by Normative Instruction (NI) No. 161. The prevalence of noncompliant samples, as indicated by the legislation, is a disconcerting finding that highlights the potential risks to public health associated with the sale of these cheeses. Consequently, the implementation of more stringent monitoring measures regarding the informal trade of fresh cheeses becomes imperative to mitigate the potential contribution to foodborne illness outbreaks.

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Published

2024-12-19

How to Cite

SÁ, A. C. de, TADIELO, L. E., SANTOS, E. A. R. dos, CERQUEIRA-CÉZAR, C. K., CHINAQUE, L. C., JULIANO, L. C. B., OLIVEIRA, J. P. de, SILVA, E. C. da, FLAMÍNIO, A. P., MARTINS, O. A., MIONI, M. de S. R., PAES, A. C., POSSEBON, F. S., & PEREIRA, J. G. (2024). Listeria monocytogenes, Coxiella burnetii, and Bacillus cereus in fresh cheese illegally marketed without inspection. Food Science and Technology, 44. https://doi.org/10.5327/fst.000381

Issue

Vol. 44 (2024)

Section

Original Articles