Optimization of bioprocesses of Lactiplantibacillus plantarum UFSJP2 with antioxidant activity and its viability in graviola (Annona muricata) sorbet

Heloísa Caneiro COLARES; Vinícius Souza TARABAL; Camila Ismar da SILVA; Hiure Gomes Ramos MEIRA; Marina Marques Lopes de Morais TOLEDO; Pedro Pires Goulart GUIMARÃES; Adriano Guimarães PARREIRA; José Antonio da SILVA; Daniel Bonoto GONÇALVES; Juliana Teixeira de MAGALHÃES; Paulo Afonso GRANJEIRO

doi:10.5327/fst.00373

Authors

Heloísa Caneiro COLARES Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0002-3354-5774
Vinícius Souza TARABAL Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0002-8736-8531
Camila Ismar da SILVA Universidade Federal de São João del-Rei, Microbiology Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0009-0002-2835-2502
Hiure Gomes Ramos MEIRA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0001-8563-3170
Marina Marques Lopes de Morais TOLEDO Universidade Federal de São João del-Rei, Cellular Biochemistry Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0002-9273-7389
Pedro Pires Goulart GUIMARÃES Universidade Federal de Minas Gerais, Department of Physiology and Biophysics, Belo Horizonte, MG, Brazil. https://orcid.org/0000-0003-4534-2779
Adriano Guimarães PARREIRA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0002-7770-7165
José Antonio da SILVA Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0001-9134-1211
Daniel Bonoto GONÇALVES Universidade Federal de São João del-Rei, Department of Biosystems Engineering, São João del-Rei, MG, Brazil. https://orcid.org/0000-0002-8178-1026
Juliana Teixeira de MAGALHÃES Universidade do Estado de Minas Gerais, Microbiology Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0002-0532-7323
Paulo Afonso GRANJEIRO Universidade Federal de São João del-Rei, Biotechnological Processes and Macromolecules Purification Laboratory, Divinópolis, MG, Brazil. https://orcid.org/0000-0003-0322-0861

DOI:

https://doi.org/10.5327/fst.00373%20

Keywords:

probiotics, bioprocess, non-dairy

Abstract

Functional foods within a varied diet at effective dosages may confer health benefits that surpass the scope of basic nutrition, with probiotics assuming a significant role in this domain. The use of sorbet enriched with probiotics is an alternative to dairy products. However, the viable bioprocessing for probiotic production presents substantial challenges. The aim of this study was to optimize the bioprocesses of Lactiplantibacillus plantarum UFSJP2 and to evaluate its antioxidant activity and viability in graviola (Annona muricata) sorbet. The optimized production of UFSJP2 biomass was 787% greater than non-optimized processes, resulting in a yield of 3.7 x 10¹⁰ CFU.mL^-1 under conditions comprising 10.55 g.L^-1 yeast extract, 10 g.L^-1 peptone, and 0.16 g.L^-1 manganese sulfate. The aggregated spherical structure observed in SEM may have a certain protective effect. Antioxidant activity assays revealed efficacies of 79% and 46% for intact and lysed cells, respectively. The survival rate of the UFSJP2 strain in the presence of hydrogen peroxide was observed at 77.5% (1.0 mM for 8 h). Notably, there was no diminution in the number of viable cells of UFSJP2 in graviola sorbet in 30 days. These results underscore a cost-effective strategy for probiotic production and for enhancing viability within plant-based matrices.

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Optimization of bioprocesses of Lactiplantibacillus plantarum UFSJP2 with antioxidant activity and its viability in graviola (Annona muricata) sorbet

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