Association between coa gene and enterotoxin gene in S. aureus from dairy cattle in Brazil

Authors

  • Mylena Karoline VALMORBIDA Universidade Estadual Paulista “Júlio de Mesquita Filho”, Postgraduate Program in Agricultural Microbiology, Faculty of Agricultural and Veterinary Sciences, Department of Veterinary Pathology, Jaboticabal, SP, Brazil. https://orcid.org/0000-0002-8154-2420
  • Marita Vedovelli CARDOZO Universidade do Estado de Minas Gerais, Department of Biomedical Sciences and Health, Passos, MG, Brazil. https://orcid.org/0000-0003-3972-0198
  • Camila Chioda de ALMEIDA Universidade Estadual Paulista “Júlio de Mesquita Filho”, Postgraduate Program in Agricultural Microbiology, Faculty of Agricultural and Veterinary Sciences, Department of Veterinary Pathology, Jaboticabal, SP, Brazil. https://orcid.org/0000-0001-5454-5426
  • Natália PEREIRA Universidade Estadual Paulista “Júlio de Mesquita Filho”, Postgraduate Program in Agricultural Microbiology, Faculty of Agricultural and Veterinary Sciences, Department of Veterinary Pathology, Jaboticabal, SP, Brazil. https://orcid.org/0000-0001-5914-084X
  • Diogenes DEZEN Instituto Federal Catarinense, Veterinary Microbiology Laboratory, Concórdia, SC, Brazil. https://orcid.org/0000-0002-0941-9954
  • Marcella Zampoli de ASSIS Instituto Federal Catarinense, Veterinary Microbiology Laboratory, Concórdia, SC, Brazil. https://orcid.org/0000-0001-8860-4914
  • Newton Valério VERBISCK Empresa Brasileira de Pesquisa Agropecuária, Embrapa beef cattle, Campo Grande, MS, Brazil. https://orcid.org/0000-0001-9817-7223
  • Eliete GRIEBELER Instituto Federal Catarinense, Veterinary Microbiology Laboratory, Concórdia, SC, Brazil. https://orcid.org/0000-0002-5198-6805
  • Lucas José Luduverio PIZAURO Universidade Estadual de Santa Cruz, Department of Agricultural and Environmental Sciences, Ilhéus, BA, Brazil. https://orcid.org/0000-0002-1071-8740
  • Fernando Antônio de ÁVILA Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculty of Agricultural and Veterinary Sciences, Department of Preventive Veterinary Medicine and Animal Reproduction, Jaboticabal, SP, Brazil. https://orcid.org/0000-0002-9779-2213

DOI:

https://doi.org/10.5327/fst.16222

Keywords:

mastitis, toxins, resistance, pulsed-field gel electrophoresis, dairy

Abstract

Staphylococcus aureus is an important agent in bovine mastitis, and some specific virulence factors may be implicated in this disease. Therefore, this study aimed to investigate the importance of the presence of coagulase, superantigens, genotypic and phenotypic resistance, and pulsotypes in 65 S. aureus isolates from bovine clinical and subclinical mastitis in the Southeast of Brazil. A high correlation was observed between the genes coa and see, as well as between the sei and the see and seh. High resistance rates were observed for penicillin (95.4%), tetracycline (89.2%), cefoxitin (86.1%), oxacillin (84.6%), erythromycin (84.6%), clindamycin (84.6%), chloramphenicol (81.5%), ceftriaxone (80.0%), and ampicillin (80.0%). Analysis of antimicrobial resistance profiles showed that 89.2% of isolates were multi-drug-resistant. No mecA-positive S. aureus isolates were detected. It was observed that seven isolates were resistant to all the β-lactam tested while being susceptible to cefoxitin, which could be indicative of borderline methicillin resistance in S. aureus⁠. High genetic diversity with no specific virulence profile being predominant was observed. Thus, this study observed a high correlation between the coa and enterotoxins genes, and demonstrates that there is no predominant pulsotype causing intramammary infection and that there is a high rate of antibiotic resistance in S. aureus isolates from dairy farms in the southeast regions of Brazil.

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Published

2023-10-05

How to Cite

VALMORBIDA, M. K., CARDOZO, M. V., ALMEIDA, C. C. de, PEREIRA, N., DEZEN, D., ASSIS, M. Z. de, VERBISCK, N. V., GRIEBELER, E., PIZAURO, L. J. L., & ÁVILA, F. A. de. (2023). Association between coa gene and enterotoxin gene in S. aureus from dairy cattle in Brazil. Food Science and Technology, 43. https://doi.org/10.5327/fst.16222

Issue

Vol. 43 (2023)

Section

Original Articles