Effects of cold storage and freezing on sheep’s milk
DOI:
https://doi.org/10.5327/fst.00137%20Keywords:
cold storage, dairy microbiology, milk quality, ovine milk, preservationAbstract
Sheep’s milk production in Brazil is focused on the industrialization of high added value derivatives. Some factors, such as seasonal production, short lactation period, and microbiological contamination, cause a shortage of this raw material throughout the year. It is well known that low temperatures are used in the conservation of dairy products and the objective of this study was to verify the microbiological and physical-chemical quality in refrigerated and frozen raw sheep’s milk. Standard plate counts, psychrotrophics, Staphylococcus aureus, total coliforms, and fecal coliforms, as well as titratable acidity, pH, and water activity were analyzed in samples 1 day after refrigeration and 8 and 15 days after freezing temperatures. Overall, the results of refrigerated milk were based on the legislation, but frozen milk showed high values of aerobic plate, S. aureus, and acidity, especially after 15 days of storage. Psychrotrophics and coliform counts were also high, indicating a risk for the quality of the raw milk and derivatives, which emphasizes the importance of handler training for good practices in the farm, as well as in the dairy industry. Freezing may be a viable strategy; however, additional studies testing lower temperatures and previous milk pasteurization are necessary to guarantee milk quality and consumer safety.
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References
Basanisi, M., Nobili, G., La Bella, G., Russo, R., Spano, G., Normanno, G., & La Salandra, G. (2016). Molecular characterization of Staphylococcus aureus isolated from sheep and goat cheeses in southern Italy. Small Ruminant Research, 135, 17-19. https://doi.org/10.1016/j.smallrumres.2015.12.024
Biegalski, J., Cais-Sokolińska, D., Tomaszewska-Gras, J., & Baranowska, H. M. (2021). The Effect of freezing sheep’s milk on the meltability, texture, melting and fat crystallization profiles of fresh pasta filata cheese. Animals, 11(9), 2740. https://doi.org/10.3390/ani11092740
Bishop, S., & Morris, C. (2007). Genetics of disease resistance in sheep and goats. Small Ruminant Research, 70(1), 48-59. https://doi.org/10.1016/j.smallrumres.2007.01.006
Bottiroli, R., Zhang, C., Aprea, E., Fogliano, V., Hettinga, K., & Gasperi, F. (2020). Short-time freezing does not alter the sensory properties or the physical stability of ultra-high-temperature hydrolyzed-lactose milk. Journal of Dairy Science, 103(10), 8822-8828. https://doi.org/10.3168/jds.2020-18415
Brasil (2018). Regulamentos Técnicos que fixam a identidade e as características de qualidade que devem apresentar o leite cru refrigerado, o leite pasteurizado e o leite pasteurizado tipo A (Instrução Normativa nº 76, 26 nov. 2018). Diário Oficial da União.
Brasil (2022). Manual de Métodos Oficiais para Análise de Alimentos de Origem Animal. MAPA.
Clark, J. T. (2016). Processing of frozen sheep milk: current procedures and difficulties encountered. Retrieved from www.ansci.wisc.edu/extensionew%20copy/sheep/Publications_and_Proceedings/symposium_04/pdf%20of%20Dairy%20Sheep%20Proceedings/clark%20frozen%20sheep%20milk%20edited%209-23-04%20Proc.pdf
European Commission (2004). Laying down specific hygiene rules for food of animal origin (Regulation (EC) No 853/2004, 29 April 2004. Official Journal of the European Union. Retrieved from https://eurlex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2004:139:0055:0205:en:PDF
Fava, L. W., Külkamp-Guerreiro, I. C., & Pinto, A. T. (2014). Rendimento de coalhada obtida a partir de leite fresco, resfriado e congelado de ovelhas da raça Lacaune e caracterização física do soro obtido. Ciência Rural, 44(5), 937-942. https://doi.org/10.1590/S0103-84782014000500028
Ferreira, M. B., Nespolo, C. R., Centenaro, G. S., Messa, S. P., Farias, A. C. R., & Stefani, L. M. (2021). Innovative dulce de leche made by sheep’s milk with and without the addition of sheep’s milk cream. Food Science and Technology, 41(Suppl. 1), 65-71. https://doi.org/10.1590/fst.11120
Flis, Z., & Molik, E. (2021). Importance of bioactive substances in sheep’s milk in human health. International Journal of Molecular Sciences, 22(9), 4364. https://doi.org/10.3390/ijms22094364
Forsythe, S. J. (2019). The Microbiology of Safe Food. 3. ed. Wiley.
Frece, J., Vrdoljak, M., Filipčić, M., Jelić, M., Čanak, I., Jakopović, Ž., Pleadin, J., Gobin, I., Dragičević, T. L., & Markov, K. (2016). Microbiological quality and variability of natural microbiota in Croatian cheese maturing in lambskin sacks. Food Technology and Biotechnology, 54(2), 129-134. https://doi.org/10.17113/ftb.54.02.16.4418
Gavião, E. R., Munieweg, F. R., Czarnobay, M., Dilda, A., Stefani, L. C. M., & Nespolo, C. R. (2020). Development and characterization of two novel formulations of Labneh cheese of sheep’s milk. Food Science and Technology, 41(3), 708-715. https://doi.org/10.1590/fst.20020
Khayrullin, M., & Rebezov, M. (2023). Study on the effects of different sterilization methods and storage conditions on milk quality. Food Science and Technology, 43, e53421. https://doi.org/10.5327/fst.53421
Malissiova, E., Papadopoulos, T., Kyriazi, A., Mparda, M., Sakorafa, C., Katsioulis, A., Kyritsi, M., Zdragas, A., & Hadjichristodoulou, C. (2017). Differences in sheep and goats milk microbiological profile between conventional and organic farming systems in Greece. Journal of Dairy Research, 84(2), 206-213. https://doi.org/10.1017/S0022029917000103
Malta, D. S., Dalmina, E. M., Schmeier, M. N., Seguenka, B., Steffens, J., Bianchi, A. E., Tribst, A. A. L., Cavalheiro, D., & Rigo, E. (2021). Impact of the preservation methods of sheep milk on the characteristics of Requeijão cremoso processed cheese. International Dairy Journal, 121, 105101. https://doi.org/10.1016/j.idairyj.2021.105101
Martin, N. H., Trmčić, A., Hsieh, T.-H., Boor, K. J., & Wiedmann, M. (2016). The evolving role of coliforms as indicators of unhygienic processing conditions in dairy foods. Frontiers in Microbiology, 7, 1549. https://doi.org/10.3389/fmicb.2016.01549
MeterGroup (2023). Aqualab Series 4TE -Atividade de Água por ponto de orvalho. Retrieved from http://metergroup.com.br/aqualab/produtos/4te-ponto-de-orvalho
Mohapatra, A., Shinde, A. K., & Singh, R. (2019). Sheep milk: A pertinent functional food. Small Ruminant Research, 181, 6-11. https://doi.org/10.1016/j.smallrumres.2019.10.002
Morel, J., Robertson, L., & Archer, R. (2020). Rapid freezing of sheep Milk. Food New Zealand, 20(1), 15-17.
Munieweg, F. R., Gavião, E. R., Czarnobay, M., Dilda, A., Stefani, L. C. M., & Nespolo, C. R. (2021). Mascarpone cheese from sheep's milk-a new option for the consumer. Food Science and Technology, 41(Suppl. 2), 568-575. https://doi.org/10.1590/fst.32420
Munieweg, F. R., Nespolo, C. R., Pinheiro, F. C., Gavião, E. R., Pinheiro, F. C., & Czarnobay, M. (2017). Qualidade do leite cru ovino armazenado sob refrigeração. Vigilância Sanitária em Debate, 5(1), 52-59. https://doi.org/10.22239/2317-269X.00848
Nespolo, C. R., Oliveira, F. A., Pinto, F. S. T., & Olivera, F. C. (2015). Práticas em Tecnologia de Alimentos. Artmed.
Park, Y., Lee, J., & Lee, S. (2006). Effects of frozen and refrigerated storage on organic acid profiles of goat milk plain soft and Monterey jack cheeses. Journal of Dairy Science, 89(3), 862-871. https://doi.org/10.3168/jds.s0022-0302(06)72150-6
Rocha Júnior, R. L., Santos Silva, F. D., Pinto, D. D. C., Costa, R. L., Gomes, H. B., Herdies, D. L., Freitas, I. G. F., & Nova, T. S. V. (2022). Análise de extremos de temperatura no Sul do Brasil. Revista Brasileira de Climatologia, 30(18), 445-460. https://doi.org/10.55761/abclima.v30i18.14857
Silva, N. D., Junqueira, V. C. A., Arruda Silveira, N. F., Taniwaki, M. H., Gomes, R. A. R., & Okazaki, M. M. (2017). Manual de Métodos de Análise Microbiológica de Alimentos e Água (5. ed.). Varela.
Taffarel, L. E., Costa, P. B., Tsutsumi, C. Y., Klosowski, E. S., Portugal, E. F., & Lins, A. C. (2015). Variação da composição e qualidade do leite em função do volume de produção, período do ano e sistemas de ordenha e de resfriamento. Semina: Ciências Agrárias, 36(Suppl. 1), 2287-2299. https://doi.org/10.5433/1679-0359.2015v36n3Supl1p2287
Theodoridis, A., Vouraki, S., Morin, E., Koutouzidou, G., & Arsenos, G. (2022). Efficiency Analysis and Identification of Best Practices and Innovations in Dairy Sheep Farming. Sustainability, 14(21), 13949. https://doi.org/10.3390/su142113949
Tonamo, A., Komlósi, I., Varga, L., Czeglédi, L., & Peles, F. (2020). Bacteriological quality of raw ovine milk from different sheep farms. Animals, 10(7), 1163. https://doi.org/10.3390/ani10071163
Tribst, A., Falcade, L., & Oliveira, M. (2019). Strategies for raw sheep milk storage in smallholdings: Effect of freezing or long-term refrigerated storage on microbial growth. Journal of Dairy Science, 102(6), 4960-4971. https://doi.org/10.3168/jds.2018-15715