Molecular analysis of Toxoplasma gondii in samples of açaí (Euterpe oleracea) ice cream purchased from commercial establishments
DOI:
https://doi.org/10.5327/fst.00434Palavras-chave:
Euterpe oleracea, açai, toxoplasmosis, diagnosis, spatial analysisResumo
The purpose of this study was to analyze the occurrence of Toxoplasma gondii in the samples of ice cream made with açai (Euterpe oleracea) pulp. Fifty samples of ice cream made with açaí pulp, purchased from 11 municipalities in the state of São Paulo, were analyzed. Conventional polymerase chain reaction (PCR) was performed with the primers TOX4/TOX5, and nested PCR (nPCR) was carried out with the primers TgNP1/TgNP2. Of the 50 samples evaluated, 1 (2%) was positive according to nPCR. The molecular detection of T. gondii in the samples of ice cream made with açai pulp in this study serves as an alert of the need for continuing epidemiological vigilance to detect this zoonosis, whose occurrence is increasing due to the widespread and expanding consumption of food products made from açai pulp.
Downloads
Referências
Attias, M., Teixeira, D. E., Benchimol, M., Vommaro, R. C., Crepaldi, P. H., Sozua, W. (2020). The life-cycle of Toxoplasma gondii reviewed using animations. Parasites & Vectors, 13, 588. https://doi.org/10.1186/s13071-020-04445-z
Daguer, H., Vicente, R. T., da Costa, T., Virmond, M. P., Hamann, W., & Amendoeira, M. R. R. (2004). Soroprevalência de anticorpos anti-Toxoplasma gondii em bovinos e funcionários de matadouros da microrregião de Pato Branco, Paraná, Brasil. Ciência Rural, 34(4), 1133-1137. https://doi.org/10.1590/S0103-84782004000400026
Dehkordi, F. S., Borujeni, M. R., Rahimi, E., & Abdizadeh, R. (2013). Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran. Foodborne Pathogens and Disease, 10(2), 120-125. https://doi.org/10.1089/fpd.2012.1311
de Moura, L., Bahia-Oliveira, L. M., Wada, M. Y., Jones, J. L., Tuboi, S. H., Carmo, E. H., Ramalho, W. M., Camargo, N. J., Trevisan, R., Graça, R. M., da Silva, A. J., Moura, I., Dubey, J. P., & Garrett, D. O. (2006). Waterborne toxoplasmosis, Brazil, from field to gene. Emerging Infectious Diseases, 12(2), 326-329. https://doi.org/10.3201/eid1202.041115
Dumètre, A., & Dardè, M. L. (2003). How to detect Toxoplasma gondii oocysts in environmental samples? FEMS Microbiology Reviews, 27(5), 651-661. https://doi.org/10.1016/s0168-6445(03)00071-8
Ekman, C. C. J. E., Chiossi, M. F. V., Meireles, L. R., Andrade Júnior, H. F., Figueiredo, W. M., Marciano, M. A. M., & Luna, E. J. A. (2012). Case-control study of an outbreak of acute toxoplasmosis in an industrial plant in the state of São Paulo, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, 54(5), 239-244. https://doi.org/10.1590/s0036-46652012000500001
Homan, W. L., Vercammen, M., Braekeleer, J., & Verschueren, H. (2000). Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. International Journal for Parasitology, 30(1), 69-75. https://doi.org/10.1016/s0020-7519(99)00170-8
Hurtado, A., Moreno, G. A. B., Barandika, J., & Pérez, A. L. G. (2001). Single tube nested PCR for the detection of Toxoplasma gondii in fetal tissues from naturally aborted ewes. Veterinary Parasitology, 102(1-2), 17-27. https://doi.org/10.1016/s0304-4017(01)00526-x
Jeffers, V., Tampaki, Z., Kim, K., & Sullivan Jr., W. J. (2018). A latent ability to persist: Differentiation in Toxoplasma gondii. Cellular and Molecular Life Sciences, 75, 2355-2373. https://doi.org/10.1007/s00018-018-2808-x
Kochanowski, J. A., & Konshy, A. A. (2018). Toxoplasma gondii. Current Biology, 28(15), R761-R783. https://doi.org/10.1016/j.cub.2018.06.016
Landis, R. J., & Koch, G. G. (1977). The measurement of observer agreement for categorical data. Biometrics, 33(1), 159-174. https://doi.org/10.2307/2529310
Marchioro, A. A., Tiyo, B. T., Colli, C. M., de Souza, C. Z., Garcia, J. L., Gomes, M. L., & Falavigna-Guilherme, A. L. (2016). First detection of Toxoplasma gondii DNA in the fresh leafs of vegetables in South America. Vector Borne and Zoonotic Diseases, 16(9), 624-626. https://doi.org/10.1089/vbz.2015.1937
Marín-García, P. J., Planas, N., & Llobat, L. (2022). Toxoplasma gondii in foods: Prevalence, control, and safety. Foods, 11, 2542. https://doi.org/10.3390/foods11162542
Meireles, L. R., Ekman, C. C., Andrade J. R. H. F., & Luna, E. J. (2015). Human toxoplasmosis outbreaks and the agent infecting form: Findings from a systematic review. Revista do Instituto de Medicina Tropical de São Paulo, 57(5), 369-376. https://doi.org/10.1590/s0036-46652015000500001
Milne, G., Webster, J. P., & Walker, M. T. (2020). Toxoplasma gondii: An underestimated threat? Trends in Parasitology, 36(12), 959-969. https://doi.org/10.1016/j.pt.2020.08.005
Montazer, I., Moradi, E., Moosazadeh, M., Hosseini, S. H., & Fakhar, M. (2023). Relationship between Toxoplasma gondii infection and psychiatric disorders in Iran: A systematic review with meta-analysis. PLoS One, 18(8), e0284954. https://doi.org/10.1371/journal.pone.0284954
Morais, B. R. dos A. P., Freire, A. B. C., Barbosa, D. R. L., da Silva, L. de C. T., Pinheiro, A. F., da Costa, S. S., Ramos, F. L. de P., Bichara, C. N. C., Lima, L. J. B., da Silva, A. V., de Souza, S. R. P., Piqueira Neto, L. P., Gonçalves, N. V., Póvoa, M. M., & do Carmo, E. L. (2016). Surto de toxoplasmose aguda no Município de Ponta de Pedras, Arquipélago do Marajó, Estado do Pará, Brasil: Características clínicas, laboratoriais e epidemiológicas. Revista Pan-Amazônica de Saúde, 7(esp), 143-152. https://doi.org/10.5123/s2176-62232016000500016
Pereira, K. S., Franco, R. M., & Leal, D. A. (2010). Transmission of toxoplasmosis (Toxoplasma gondii) by foods. Advances in Food and Nutrition Research, 60, 1-19. https://doi.org/10.1016/S1043-4526(10)60001-0
Sah, R. P., Hasanuzzaman, T. M., Anisur, R. A. K. M., Alama, M. Z., & Talukder, M. H. (2019). Seroprevalence of Toxoplasma gondii infection in ruminants in selected districts in Bangladesh. Veterinary Parasitology: Regional Studies and Reports, 11, 1-5. https://doi.org/10.1016/j.vprsr.2017.10.008
Santos Silva, A. C., de Barros, L. D., Barros, V. M. C., Alcântara, A. M., Andrade, M. R., Garcia, J. L., Mota, R. A., & Porto, W. J. N. (2020). Occurrence of atypical and new genotypes of Toxoplasma gondii in free-range chickens intended for human consumption in Brazil. Acta Parasitologica, 65, 774-778. https://doi.org/10.2478/s11686-020-00194-2
Symeonidou, I., Sioutas, G., Lazou, T., Gelasakis, A. I., & Papadopoulos, E. (2023). A review of Toxoplasma gondii in animals in Greece: A foodborne pathogen of public health importance. Animals, 13(15), 2530. https://doi.org/10.3390/ani13152530
Zhang, S., Wu, G., Shi, Y., Liu, T., Xu, L., Dai, Y., Chang, W., & Ma, X. (2022). Understanding etiology of community-acquired central nervous system infections using metagenomic next-generation sequencing. Frontiers in Cellular and Infection Microbiology, 12, 979086. https://doi.org/10.3389/fcimb.2022.979086
Zhao, X. Y., & Ewald, S. E. (2020). The molecular biology and immune control of chronic Toxoplasma gondii infection. Journal of Clinical Investigation, 130(7), 3370-3380. https://doi.org/10.1172/jci136226