Effects of plant growth-promoting bacteria on the performance of chickpea seedlings

Autores

  • Ruan da Silva CÂNDIDO Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0009-0000-3955-990X
  • Patrícia Costa SILVA Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0000-0002-8894-1512
  • Josué Gomes DELMOND Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0000-0002-1099-9204
  • Pedro Rogerio GIONGO Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0000-0002-9042-9120
  • Eduardo Silva COUTO Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0009-0005-0715-3864
  • Lucas Inácio da SILVA Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0009-0007-4003-8192
  • Ana Flávia Alves FERREIRA Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0009-0000-7442-3135
  • Emanuelly Silva ARAÚJO Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0009-0002-3452-8201
  • Carlos Miguel Barbosa VALÉRIO Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0009-0005-1039-241X
  • Adriana Rodolfo da COSTA Universidade Estadual de Goiás, Campus Sudoeste, Unidade Universitária de Santa Helena de Goiás, Departamento de Engenharia Agrícola, Santa Helena de Goiás, GO, Brazil. https://orcid.org/0000-0002-0263-3309

DOI:

https://doi.org/10.5327/fst.00415

Palavras-chave:

Cicer arietinum L., Rhizobacteria, bioinputs, root mass accumulation

Resumo

Using plant growth-promoting bacteria (PGPB) is a sustainable option for the cultivation of chickpeas as well as other crops, and their effects can be observed from the beginning of development. The objective of this study was to evaluate the development of the seedlings of chickpea cultivars under inoculation with PGPB. The experiment was conducted in a greenhouse, and the experimental design adopted was completely randomized in a 2 × 3 factorial scheme (2: application or not of the inoculant in chickpea seed treatment; 3 chickpea cultivars: BRS Aleppo, BRS Cícero, and BRS Toro). In this research, it was observed that growth-promoting bacteria showed positive effects in the first days of chickpea development, having a more significant effect on the growth and accumulation of root mass. The authors suggested that biomass accumulation in the root system is due to the secretion of substances by microorganisms inoculated via seeds, which promote root development and consequently greater absorption of nutrients. The BRS Aleppo cultivar showed better performance in terms of seedling emergence, but the seedlings had lower biomass accumulation in both shoots and roots. The use of PGPB promoted fast and early chickpea growth.

Downloads

Não há dados estatísticos.

Referências

Abd-Alla, M. H., Nafady, N. A., Bashandy, S. R., & Hassan, A. A. (2019). Mitigation of effect of salt stress on the nodulation, nitrogen fixation and growth of chickpea (Cicer arietinum L.) by triple microbial inoculation. Rhizosphere, 10, e100148. https://doi.org/10.1016/j.rhisph.2019.100148

Allen, R. G., Pereira, L. S., Raes, D., & Smith, M. (2006). Evapotranspiración del cultivo: guías para la determinación de los equerimientos de água de los cultivos. Food and Agriculture Organization.

Almeida Neta, M. N., Almeida, E. S., Costa, C. A., Nunes, J. A. R., Fernandes, L. A., & Pegoraro, R. F. (2021). Inoculation of Bacillus spp. and nitrogen levels increase chickpea production. Ciência & Agrotecnologia, 45, e015421. https://doi.org/10.1590/1413-7054202145015421

Alvares, C. A., Stape, J. L., Sentelhas, P. C., Gonçalves, J. D. M., & Sparovek, G. (2013). Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift, 22(6), 711-728. https://doi.org/10.1127/0941-2948/2013/0507

Amaro, H. T. R., David, A. M. S.S., Assis, M. O., Rodrigues, B. R. A., Cangussú, L. V. S., & Oliveira, M. B. (2015). Testes de vigor para avaliação da qualidade fisiológica de sementes de feijoeiro. Revista de Ciências Agrárias, 38(3), 383-389. https://doi.org/10.19084/rca.16943

Araújo, E. F. L., & Souza, E. R. B. (2018). Phenology and reproduction of Campomanesia adamantium (Cambess.) O. Berg (Myrtaceae). Scientific Electronic Archives, 11(2), 166-175. https://doi.org/10.36560/1122018414

Balbinot, W. G., Rodrigues, S., & Botelho, G. R. (2020). Isolates of Bacillus sp. from garlic: effect on corn development and plant growth-promoting mechanisms. Revista Brasileira de Ciência do Solo, 44, e0200043. https://doi.org/10.36783/18069657rbcs20200043

Brasil (2009). Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Regras para análise de sementes. MAPA/ACS.

Carvalho, S. I. C., Bianchetti, L. B., Silva, P. P., & Nascimento, W. M. (2021). Fenologia do grão-de-bico tipo Kabuli. Comunicado Técnico, 133. Embrapa.

Cassán, F., Coniglio, A., López, G., Molina, R., Nievas, S., Carlan, C. L. N., Donadio, F., Torres, D., Rosas, S., Pedrosa, F.O., Souza, E., Zorita, M. D., De-Bashan, L., & Mora, V. (2020). Everything you must know about Azospirillum and its impact on agriculture and beyond. Biology and Fertility of Soils, 56(1), 461-479. https://doi.org/10.1007/s00374-020-01463-y

Castilho, I. M., Catão, H. C. R. M., Caixeta, F., Marinke, L. S., Martins, G. Z., & Menezes, J. B. C. (2019). Teste de condutividade elétrica na avaliação do potencial fisiológico de sementes de grão-de-bico. Revista de Ciências Agrárias, 42(3), 691-697. https://doi.org/10.19084/rca.17449

Chang, L., Lan, Y., Bandillo, N., Ohm, J. B., Chen, B., & Rao, J. (2022). Plant proteins from green pea and chickpea: Extraction, fractionation, structural characterization and functional properties. Food Hydrocolloids, 123, e107165. https://doi.org/10.1016/j.foodhyd.2021.107165

Dias, L. B. X., Queiroz, P. A. M., Ferreira, L. B. S., Santos, W. V., Freitas, M. A. M., Silva, P. P., Nascimento, W. M., & Araújo, E. F. L. (2019). Teste de condutividade elétrica e embebição de sementes de grão-de-bico. Revista Brasileira de Ciências Agrárias, 14(2), e5641. https://doi.org/10.5039/agraria.v14i2a5641

Faria, A. V., Barbosa, K. P., Costa, A. R., Silva, P. C., Drumond, A. A. L., França, J. B. A., & Silva, G. M. (2021). Profundidad de siembra y tamaño de las semillas: desarrollo de las plántulas de frijol mungo. Revista de Investigación Agraria y Ambiental, 12(2), 13-24. https://doi.org/10.22490/21456453.3833

Ferreira, D. F. (2019). SISVAR: a computer analysis system to fixed effects split plot type designs. Revista Brasileira de Biometria, 37(4), 529-535. https://doi.org/10.28951/rbb.v37i4.450

Freire, M. H. C., Sousa, G. G., Souza, M. V. P., Ceita, E. D. R., Fiusa, J. N., & Leite, K. N. (2018). Emergence and biomass accumulation in seedlings of rice cultivars irrigated with saline water. Revista Brasileira de Engenharia Agrícola e Ambiental, 22(7), 471-475. https://doi.org/10.1590/1807-1929/agriambi.v22n7p471-475

Hammer, O., Harper, D. A. T., & Ryan, P. D. (2001). PAST: Paleontological statistics software package for education and analysis. Palaentologia Electronica, 4(1), 1-9. https://paleo.carleton.ca/2001_1/past/past.pdf

Hosken, B. C. S., Costa, C. A., Nascimento, W. M., Santos, L. D. T., Mendes, R. B., & Menezes, J. B. C. (2017). Productivity and quality of chickpea seeds in Northern Minas Gerais, Brazil. Revista Brasileira de Ciências Agrárias, 12(3), 261-268. https://doi.org/10.5039/agraria.v12i3a5445

Hosseini, N. M., Siddique, K. H. M., Palta, J. A., & Berger, J. (2009). Effect of soil moisture content on seedling emergence and early growth of some chickpea (Cicer arietinum l.) genotypes. Journal of Agricultural Science and Technology, 11(4), 401-411. http://jast.modares.ac.ir/article-23-10807-en.html

Joshi, D., Chandra, R., Suyal, D. C., Kumar, S., & Goel, R. (2019). Impacts of bioinoculants Pseudomonas jesenii MP1 and Rhodococcus qingshengii S10107 on chickpea (Cicer arietinum L.) yield and soil nitrogen status. Pedosphere, 29(3), 388-399. https://doi.org/10.1016/S1002-0160(19)60807-6

Kaur, R., & Prasad, K. L. (2021). Nutritional characteristics and value-added products of Chickpea (Cicer arietinum) - A review. Journal of Postharvest Technology, 9(2), 1-13. Retrieved from https://acspublisher.com/journals/index.php/jpht/article/view/15170

Magruire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2(2), 176-177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x

Maleki, A., Pournajaf, M., Naseri, R., Rashnavadi, R., & Heydari, M. (2014). The effect of supplemental irrigation, nitrogen levels and inoculation with Rhizobium bacteria on seed quality of chickpea (Cicer arietinum L.) under rainfed conditions. International Journal of Current Microbiology Applied Sciences, 3(6), 902-909. Retrieved from http://www.ijcmas.com/vol-3-6/Abbas%20Maleki,%20et%20al.pdf

Matthews, S., El-Khadem, R., Casarini, E., Khajed-Hosseini, M., Nasehzadeh, M., & Wagner, M. H. (2010). Rate of physiological germination compared with the cold test and accelerated ageing as a repeatable vigour test for maize. Seed Science and Technology, 38(2), 379-389. https://doi.org/10.15258/sst.2010.38.2.11

Mukherjee, A., Singh, B., & Verma, J. P. (2020). Harnessing chickpea (Cicer arietinum L.) seed endophytes for enhancing plant growth attributes and bio-controlling against Fusarium sp. Microbiological Research, 237, e126469. https://doi.org/10.1016/j.micres.2020.126469

Nakagawa, J. (1999). Testes de vigor baseados no desempenho das plântulas. In F. C. Krzyzanowski, R. D. Vieira & J. B. França-Neto (1999). Vigor de sementes: conceitos e testes (vol. 1, pp. 1-24). ABRATES.

Nascimento, W. M., & Silva, P. P. (2019). Grão-de-bico: nova aposta do agronegócio brasileiro. Seed News, 23(3), 18-22.

Nascimento, W. M., Silva, P. P., Artiaga, O. P., & Suinaga, F. A. (2016) Grão De Bico. In W. M. Nascimento (Ed.), Hortaliças Leguminosas (pp. 89-120). Embrapa.

Palmero, F., Fernandez, J. A., Garcia, F. O., Haro, R. J., Prasad, P. V. V., Salvagiotti, F., & Ciampitti, I. A. (2022). A quantitative review into the contributions of biological nitrogen fixation to agricultural systems by grain legumes. European Journal of Agronomy, 136, e126514. https://doi.org/10.1016/j.eja.2022.126514

Parwada, C., Parwada, T. F., Chipomho, J., Mapope, N., Chikwari, E., & Mvumi, C. (2022). Evaluation of Cicer arietinum (Chickpea) growth performance and yield in different soil types in Zimbabwe. Journal of Current Opinion in Crop Science, 3(1), 16-27. Retrieved from https://www.jcocs.com/index.php/ej/article/view/148

Pedó, T., Segalin, S. R., Silva, T. A., Martinazzo, E. G., Neto A. G., Aumonde, T. Z., & Villela, F. A. (2014). Vigor de sementes e desempenho inicial de plântulas de feijoeiro em diferentes profundidades de semeadura. Revista Brasileira de Ciências Agrárias, 9(1), 59-64. https://doi.org/10.5039/agraria.v9i1a3631

Pendergast, L., Bhattarai, S. P., & Midmore, D. J. (2019). Evaluation of aerated subsurface drip irrigation on yield, dry weight partitioning and water use efficiency of a broadacre chickpea (Cicer arietinum, L.) in a Vertosol. Agricultural Water Management, 217(5), 38-46. https://doi.org/10.1016/j.agwat.2019.02.022

Peske, S. T., Villela, F. A., & Meneghello, G. E. (2012). Sementes: fundamentos científicos e tecnológicos (3. ed.). Ed. Universitária/UFPel.

Pinheiro, R. C. (2021). Germinação, condutividade elétrica e emergência em campo na qualidade de sementes em cinco genótipos de grão de bico (Monografia de graduação, Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília).

Romanyà, J., & Casals, P. (2020). Biological nitrogen fixation response to soil fertility is species-dependent in annual legumes. Journal of Soil Science and Plant Nutrition, 20(2), 546-556. https://doi.org/10.1007/s42729-019-00144-6

Roos, E. E., & Moore III, F. D. (1975). Effect of seed coating on performance of lettuce seeds in greenhouse soil tests. Journal of the American Society for Horticultural Science, 100(5), 573-576. https://doi.org/10.21273/JASHS.100.5.573

Rosa, M. R., Dias, L. B. X., Nascimento, W. M., & Araújo, E. F. L. (2021). Standardized methodology for germination test on chickpea seeds. Multi-Science Journal, 4(1), 31-37. https://doi.org/10.33837/msj.v4i1.1371

Santos, A. P., Costa, A. R., Silva, P. C., Melo, M. C. R., & Araújo, H. L. (2017). Influência de lâminas de irrigação e fontes de nitrogênio no crescimento vegetativo do tomate cereja cultivado em ambiente protegido. Enciclopédia Biosfera, 14(25), 1-11. https://doi.org/10.18677/EnciBio_2017A65

Santos, H. G., Jacomine, P. K. T., Anjos, L. H. C., Oliveira, V.A., Lembreras, J. F., Coelho, M. R., & Cunha, T. J. F. (2018). Sistema brasileiro de classificação de solos (5. Ed.). Embrapa Solos.

Santos, M. S., Nogueira, M. A., & Hungria, M. (2019). Microbial inoculants: reviewing the past, discussing the present and previewing an outstanding future for the use of beneficial bacteria in agriculture. AMB Express, 9(25), 205-227. https://doi.org/10.1186/s13568-019-0932-0

Sharma, V., Sharma, S., Sharma, S., & Kumar, V. (2019). Synergistic effect of bio-inoculants on yield, nodulation and nutrient uptake of chickpea (Cicer arietinum L.) under rainfed conditions. Journal of Plant Nutrition, 42(4), 374-383. https://doi.org/10.1080/01904167.2018.1555850

Silva, P. C., Ferreira, A. F. A., Araújo, E. S., Bessa Neto, J. V., Costa, A. R., Fernandes, L. S., Martins, A. A. S., Cândido, R. S., Jardim, A. M. R. F., Pandorfi, H., & Silva, M. V. (2022). Cherry tomato crop management under irrigation levels: morphometric characteristics and their relationship with fruit production and quality. Gesunde Pflanzen, 75, 1277-1288. https://doi.org/10.1007/s10343-022-00770-8

Trancoso, A. C. R., Dias, D. C. F. S., Picoli, E. A. T., Silva Júnior, R. A., Silva, L. J., & Nascimento, M. N. (2021). Anatomical, histochemical and physiological changes during maturation of chickpea (Cicer arietinum L.) seeds. Revista Ciência Agronômica, 52(4), e20207534. https://doi.org/10.5935/1806-6690.20210048

Verma, G., Yadav, D. D., Kumar, A., Singh, R., Babu, S., Avasthe, R. K., Gudade, B. A., & Sharma, V. K. (2020). Impact of fertility levels and biofertilizers on root architecture, yield and nutrient uptake of chickpea (Cicer arietinum L.) crop. International Journal of Current Microbiology and Applied Science, 9(2), 2018-2024. https://doi.org/10.20546/ijcmas.2020.902.230

Xavier, G. R., Jesus, E. C., Dias, A., Coelho, M. R. R., Molina, Y. C., & Rumjanek, N. G. (2023). Contribution of biofertilizers to pulse crops: from single-strain inoculants to new technologies based on microbiomes strategies. Plants, 12(4), 954. https://doi.org/10.3390/plants12040954

Zaheer, A., Malik, A., Sher, A., Qaisrani, M. M., Mehmood, A., Khan, S. U., Ashraf, M., Mirza, Z., Karim, S., & Rasool, M. (2019). Isolation, characterization and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth. Saudi of Journal Biological Science, 26(5), 1061-1067. https://doi.org/10.1016/j.sjbs.2019.04.004

Zhang, J., Chen, W., Shang, Y., Guo, C., Peng, S. & Chen, W. (2020). Biogeographic distribution of chickpea rhizobia in the world. In V. Sharma, R. Salwan & L. K. T. Al-Ani (Eds.), Molecular aspects of plant beneficial microbes in agriculture (pp. 235-2339). Academic Press. https://doi.org/10.1016/B978-0-12-818469-1.00020-1

Downloads

Publicado

2025-03-27

Como Citar

CÂNDIDO, R. da S., SILVA, P. C., DELMOND, J. G., GIONGO, P. R., COUTO, E. S., SILVA, L. I. da, FERREIRA, A. F. A., ARAÚJO, E. S., VALÉRIO, C. M. B., & COSTA, A. R. da. (2025). Effects of plant growth-promoting bacteria on the performance of chickpea seedlings . Food Science and Technology, 45. https://doi.org/10.5327/fst.00415

Edição

Seção

Artigos Originais