Phytochemical analysis of Cagaita (Eugenia dysenterica DC) leaf extracts in Northern Minas Gerais Cerrado, Brazil
DOI:
https://doi.org/10.5327/fst.00388Palavras-chave:
Eugenia dysenterica, phytochemical screening, leaf extractsResumo
The objective of this study was to perform a phytochemical screening of the aqueous extracts of the leaves of Eugenia dysenterica DC to identify the secondary compounds present; evidence the presence of total yellow flavonoid compounds; identify total phenolic compounds, evaluate antioxidant activity; and verify the influence of the different extraction methods on the concentration of the identified secondary metabolites. For phytochemical evaluation, three aqueous extracts were obtained using infusion, decoction, and maceration. The preparation followed the methodology of Garlet (2019), with adaptations. The phytochemical screening included the determination of the total phenolic content by the FolinCiocalteu method. Absorbance was measured spectrophotometrically. Antioxidant activity was determined by oxygen radical absorption capacity method described by Prieto et al. (1999). The determination of yellow flavonoids was performed as described by Francis (1982), with modifications. Maceration was the most effective method to extract total yellow flavonoids, while decoction was the most effective to extract total phenolic compounds. Maceration resulted in higher antioxidant activity. Statistical differences indicate that the choice of extraction method should be guided by the type of bioactive compound of interest and its thermal stability to maximize the extraction of certain secondary metabolites from Eugenia Dysenterica DC.
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Figueirinha, A., Paranhos, A., Pérez-Alonso, J. J., Santos-Buelga, C., & Batista, M. T. (2008). Cymbopogon citratus leaves: Characterization of flavonoids by HPLC–PDA– ESI/MS/MS and an approach to their potential as a source of bioactive polyphenols. Food Chemistry, 110(3), 718-728. https://doi.org/10.1016/j.foodchem.2008.02.045
Fotakis, C., Tsigrimani, D., Tsiaka, T., Lantzouraki, D. Z., Strati, I. F., Makris, C., Tagkouli, D., Proestos, C., Sinanoglou, V. J., & Zoumpoulakis, P. (2016). Metabolic and antioxidant profiles of herbal infusions and decoctions. Food Chemistry, 211(3), 963-971. https://doi.org/10.1016/j.foodchem.2016.05.124
Francis, F. J. (1982). Analysis of anthocyanins in foods. In: P. Markakis (Ed.), Anthocyanins as Food Colors (pp. 181-207). Academic Press.
Garlet, T. M. B. (2019). Plantas medicinais nativas de uso popular no Rio Grande do Sul. UFSM.
Ghafoor, K., Choi, Y. H., Jeon, J.Y., & Jo, I. H. (2009). Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants, and anthocyanins from grape (Vitis vinifera) seeds. Journal of Agriculture and Food Chemistry, 57(11), 4988-4994. https://doi.org/10.1021/jf9001439
Gonçalves, R. M., Lemos, C. O. T., Leal, I. C. R., Nakamura, C. V., Cortez, D. A. G., Silva, E. A., Cabral, V. F., & Cardozo-Filho, L. (2013). Comparing conventional and supercritical extraction of (−)-Mammea A/BB and the antioxidant activity of Calophyllum brasiliense extracts. Molecules, 18(6), 6215-6229. https://doi.org/10.3390/molecules18066215
Hamed, Y. S., Abdin, M., Akhtar, H. M. S., Chen, D., Wan, P., Chen, G., & Zeng, X. (2019). Extraction, purification by macrospores resin and in vitro antioxidant activity of flavonoids from Moringa oliefera leaves. South African Journal of Botanic, 124, 270-279. https://doi.org/10.1016/j.sajb.2019.05.006
Karadag, A., Ozcelik, B., Saner S. (2009). Review of methods to determine antioxidant capacities. Food Analytical Methods, 2, 41-60. https://doi.org/10.1007/s12161-008-9067-7
Magalhães, B. E. A. & Santos, W. N. L. (2021). Capacidade antioxidante e conteúdo fenólico de infusões e decocções de ervas medicinais. In: S. Almeida Júnior (Ed.), Produtos naturais e suas aplicações: da comunidade para o laboratório (pp. 235-247). Científica Digital.
Nunes, R. F. (2018). Nanoemulsões contendo extrato aquoso das folhas de Eugenia dysenterica DC (Myrtaceae): desenvolvimento e avaliação da estabilidade, penetração cutânea e atividade antioxidante (Dissertação de Mestrado, Universidade de Brasília).
Oliveira, V. B., Zuchetto, M., Oliveira, C. F., Paula, C. S., Duarte, A. F. S., Miguel, M. D., & Miguel, O. G. (2016). Efeito de diferentes técnicas extrativas no rendimento, atividade antioxidante, doseamentos totais e no perfil por CLAE-DAD de Dicksonia sellowiana (presl.). Hook, dicksoniaceae. Revista Brasileira de Plantas Medicinais, 18(1 Suppl. 1), 230-239. https://doi.org/10.1590/1983-084X/15_106
Pérez, M. G. F., Rocha-Guzmán, N. E., Mercado-Silva, E., Loarca-Piña, G., & Reynoso-Camacho, R. (2014). Effect of chemical elicitors on peppermint (Mentha piperita) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions. Food Chemistry, 156, 273-278. https://doi.org/10.1016/j.foodchem.2014.01.101
Pinelo, M., Del Fabbro, P., Manzocco, L., Nuñez, M. J., & Nicoli, M. C. (2005). Optimization of continous phenol extraction from Viris vinifera by-products. Food Chemistry, 92(1), 109-117. https://doi.org/10.1016/j.foodchem.2004.07.015
Prado, L. C. S. (2013). Avaliação da atividade gastroprotetora do extrato aquoso das folhas de Eugenia dysenterica DC. e Campomanesia pubescens O. Berg. (Dissertação de mestrado, Universidade Federal de Uberlândia).
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum. complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337-341. https://doi.org/10.1006/abio.1999.4019
Ramalho, S. A., Nigam, N., Oliveira, G. B., Oliveira, P. A., Silva, T. O. M., Santos, A. G. P., & Narain, N. (2013). Effect of infusion time on phenolic compounds and caffeine content in black tea. Food Research International, 51(1), 155-161. https://doi.org/10.1016/j.foodres.2012.11.031
Santos, L. M. B. (2023). Alternativa verde para extração de princípios ativos das folhas de Psidium guajava L., utilizando solventes eutéticos naturais (NADES). (Trabalho de Conclusão de Curso Bacharelado Ciências Biológicas, Universidade Estadual Paulista).
Sete da Cruz, R. M., Mataruco, L., Jaski, J. M., & Barão, C. E. (2022). Extração de compostos fenólicos das folhas de ora-pro-nóbis utilizando fluidos pressurizados. 35º Congresso Latinoamericano de Química. Retrieved from https://www.abq.org.br/cbq/2022/
Soares, N. P., Santos, P. L., Vieira, V. S., Pimenta, V. S. C., & Araújo, E. G. (2016). Técnicas de prospecção fitoquímica e sua importância para o estudo de biomoléculas derivadas de plantas. Enciclopédia Biosfera, 13(24).
Souza, M. E. A. O., Gomes, M. R., Albuquerque Junior, N. M., & Candeias, V. M. S. (2021). Influência de diferentes técnicas de extração sobre a capacidade antioxidante do da casca de melancia desidratada. Research, Society and Development, 10(13), e323101321333. https://doi.org/10.33448/rsd-v10i13.21333
Souza, V. R., Pereira, P. A. P., Queiroz, S. V. B., & Carneiro, J. D. S. (2019). Determination of bioactive compounds, antioxidant activity and chemical composition of cerrado Brazilian fruits. Food Chemistry, 134(1), 381-386. https://doi.org/10.1016/j.foodchem.2012.02.191
Tomborelli, P. M., Oliveira, M. L., Oliveira, M., & Costa, D. L. M. G. (2018). Atividade antioxidante de extrato de Eugenia dysenterica a partir de diferentes frações de solventes. In: 58º Congresso Brasileiro de Química. Retrieved from https://www.abq.org.br/cbq/2018/trabalhos/10/1806-26697.html
Veber, J., Petrini, L. A., Andrade, L. B., & Siviero, J. (2015). Determinação dos compostos fenólicos e da capacidade antioxidante de extratos aquosos e etanólicos de Jambolão (Syzygium cumini L.). Revista Brasileira de Plantas Medicinais, 17(2), 267-273. https://doi.org/10.1590/1983-084X/12_181
Whaterhouse, A. L. (2002). Polyphenolics: determination of total phenolic. In R. E. Wrolstad (Ed.), Current protocols in food analytical chemistry. John Wiley & Sons.
Zanusso, P. W., Marinõ, P. A., Maldaner, G., & Gonçalves, R. P. (2023). Avaliação fitoquímica de extratos de folhas de Eugenia Uniflora L. Society and Development, 12(4), e14012441060. https://doi.org/10.33448/rsd-v12i4.41060
