The proximate composition and phytochemical screening of Momordica Balsamina (balsam apple) fruit and leaves
DOI:
https://doi.org/10.5327/fst.00177%20Palavras-chave:
Momordica balsamina, malnutrition, nutrients, proximate composition, bioactive compoundsResumo
Malnutrition is a global issue that affects both children and adults irrespective of their socio-economic status. It is therefore important to find various means to tackle malnutrition. This is especially important as undernutrition and overnutrition can be linked to a variety of non-communicable diseases (NCDs). Therefore, this study aimed to gather more insight into the nutritional and phytochemical quality of Momordica balsamina leaves and fruit (fruit pericarp, fruit flesh and seeds). The results showed that M. balsamina had a nutritional composition that would be advantageous to the human diet. The nutritional quality was verified by the presence of a high protein percentage across all samples (19.72–29.08%), with the leaves containing the highest protein content (29.08% ± 0.77). There was also a low-fat content present across all samples which ranged from 1.03% to 2.40%. The ash content indicated the presence of total minerals to be adequate (2.93–21.16%), where the pericarp had the highest ash quantity (21.16% ± 0.09). Overall, the moisture levels were low (7.11–13.40%); with M. balsamina seeds containing the highest carbohydrate content (67.84% ± 0.30).
Moreover, rich in the major phytoconstituents, M. balsamina extracts were found to contain alkaloids, saponins, cardiac glycosides, steroids and triterpenoids. Based on these findings, it can be deduced that the incorporation of M. balsamina into an individual’s diet could prevent diseases associated with malnutrition and could be used to supplement the human diet in managing certain NCDs.
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Abdulhamid, A., Jega, S. A., Sani, I., Bagudo, A. I., & Abubakar, R. (2023). Phytochemical and antibacterial activity of Mormodica balsamina leaves crude extract and fractions. Drug Discovery, 17(39), e11dd1012. https://doi.org/10.54905/disssi.v17i39.e11dd1012
Aberoumand, A., & Deokule, S. S. (2009). High nutritional value of non-conventional balsam apple (Momordica dioica Roxb.): A frica wild fruit vegetable. International Journal of Applied Agricultural Research, 4(3), 215-222.
Adegbesan, S. I. (2019). Proximate composition, qualitative and quantitative phytochemical screening of aqueous extract of Aspilia fricana (Asteraceae) C.D Adams leaves. Biomedical Journal of Scientific and Technical Research, 22, 16457-16461. https://doi.org/10.26717/BJSTR.2019.22.003711
Adhya, D., Annuario, E., Lancaster, M. A., Price, J., Baron-Cohen, S., & Srivastava, D. P. (2018). Understanding the role of steroids in typical and atypical brain development: Advantages of using a “brain in a dish” approach. Journal of Neuroendocrinology, 30(2), e12547. https://doi.org/10.1111/jne.12547
Alaekwe, I. O., Ajiwe, V. I. E., & Aguoma, C. C. (2023). Phytochemical screening and chromatographic purification of Bauhunia semibifida ROXB hexane-dichloromethane leaf extract. Journal of Materials Science and Chemical Engineering, 11(7), 14-19. https://doi.org/10.4236/msce.2023.117002
Alam, M. A., Uddin, R., Subhan, N., Rahman, M. M., Jain, P., & Reza, H. M. (2015). Beneficial role of bitter melon supplementation in obesity and related complications in metabolic syndrome. Journal of Lipids, 2015, 496169. https://doi.org/10.1155%2F2015%2F496169
Albahrani, A. A., & Greaves, R. F. (2016). Fat-Soluble Vitamins: Clinical indications and current challenges for chromatographic measurement. The Clinical Biochemistry Reviews, 37(1), 27-47.
Aleshinloye, A., Orodele, K., Adaramola, B., & Onigbinde, A. (2017). Nutritional and phytochemical analysis of ripe and unripe Roystonea regia fruit pericarp. International Journal of Multidisciplinary and Current Research, 5, 1301-1306.
Appoldt, Y., & Raihani, G. (2017). Determining moisture content. Food Quality and Safety. Retrieved from https://www.foodqualityandsafety.com/article/determining-moisture-content/?singlepage=1
Association of Official Analytical Chemist (AOAC) (1990). Official methods of analysis of the association of official’s analytical chemists (14th ed.). Association of Official Analytical Chemist.
Auwal, M. S., Saka, S., Mairiga, I. A., Sanda, K. A., Shuaibu, A., & Ibrahim, A. (2014). Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa). The Veterinary Research Forum, 5(2), 95-100.
Awad, A. M., Kumar, P., Ismail-Fitry, M. R., Jusoh, S. A., Aziz, M. F., & Sazili, A. Q. (2021). Green extraction of bioactive compounds from plant biomass and their application in meat as natural antioxidant. Antioxidants (Basel), 10(9), 1465. https://doi.org/10.3390/antiox10091465
Awode, A. U., Ezera, J. E., Adoga, S. O., & Wapwera, J. A. (2023). Phytochemical screening, antioxidant and antimicrobial activities of neem seed (Azadirachta indica) extracts. International Journal of Research and Innovation in Applied Sciences, 8, 41-47. https://doi.org/10.51584/IJRIAS.2023.8704
Azuaga, I. C., Azuaga, T. I., Ushie, O. A., Ugwuja, D. I., & Longbap, B. D. (2022). Preliminary phytochemical screening and proximate composition of ethyl acetate and hexane leaf extracts of Benn (Thaumatococcus daniellii). Journal of Research in Chemistry, 3(1), 20-23.
Bakare, R. I., Magbagbeola, O. A., Akinwande, A. I., & Okunowo, O. W. (2010). Nutritional and chemical evaluation of Momordica charantia. Journal of Medicinal Plants Research, 4, 2189-2193. https://doi.org/10.5897/JMPR10.274
Bell, L. N., & Labuza, T. P. (2000). Moisture sorption: Practical aspects of isotherm measurement and use (2nd ed.). American Association of Cereal Chemists.
Bortolotti, M., Mercatelli, D., & Polito, L. (2019). Momordica charantia, A nutraceutical approach for inflammatory related diseases. Frontiers in Pharmacology, 10, 486. https://doi.org/10.3389/fphar.2019.00486
Botelho, A. F. M., Pierezan, F., Soto-Blanco, B., & Melo, M. M. (2019). A review of cardiac glycosides: Structure, toxicokinetics, clinical signs, diagnosis and antineoplastic potential. Toxicon, 158, 63-68. https://doi.org/10.1016/j.toxicon.2018.11.429
Bulugahapitiya, V. P., & Chandani, R. P. D. S. (2002). Preliminary screening of solvent extracts of leaves, stems and root of Hemidesmus indicus for different types of secondary metabolites. In Proceedings of the First Science Symposium, Sri Lanka.
Chacha, J. S., & Laswai, H. S. (2020). Traditional practices and consumer habits regarding consumption of underutilised vegetables in Kilimanjaro and Morogoro regions, Tanzania. International Journal of Food Science, 2020, 3529434. https://doi.org/10.1155%2F2020%2F3529434
Chen, Y., Michalak, M., & Agellon, L. B. (2018). Importance of nutrients and nutrient metabolism on human health. Yale Journal of Biology and Medicine, 91(2), 95-103.
Chokki, M., Cudălbeanu, M., Zongo, C., Dah-Nouvlessounon, D., Ghinea, I. O., Furdui, B., Raclea, R., Savadogo, A., Baba-Moussa, L., Avamescu, S. M., Dinica, R. M., & Baba-Moussa, F. (2020). Exploring antioxidant and enzymes (a-amylase and b-glucosidase) inhibitory activity of Morinda lucida and Momordica charantia leaves from Benin. Foods, 9(4), 434. https://doi.org/10.3390/foods9040434
Dàvila-Hernàndez, G., Delgadillo-Arèvalo, C. S., Sànchez-Pardo, M. E., Necoechea-Mondragon, H., & Ortiz-Moreno, A. (2020). Pretreatment of tamarind pericarp to increase antioxidant availability and its application in a functional food. Journal of Food Science and Technology, 58(6), 2385-2394. https://doi.org/10.1007%2Fs13197-020-04751-5
Delimaris, I. (2013). Adverse effects associated with protein intake above the recommended dietary allowance for adults. ISRN Nutrition, 2013, 126929. https://doi.org/10.5402%2F2013%2F126929
Dey, P., Kundu, A., Kumar, A., Gupta, M., Lee, B. M., Bhakta, T., Dash, S., & Kim, H. S. (2020). Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids). Recent Advances in Natural Products Analysis, 2020, 505-567. https://doi.org/10.1016%2FB978-0-12-816455-6.00015-9
Dukhi, N. (2020). Global prevalence of malnutrition: Evidence from literature. Intechopen.
Ekor, M. (2014). The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4, 177. https://doi.org/10.3389/fphar.2013.00177
Fategbe, M. A., Avwioroko, O. J., & Ibukun, E. O. (2021). Comparative biochemical evaluation of the proximate, mineral, and phytochemical constituents of Xylopia aethiopica whole fruit, seed, and pericarp. Preventative Nutrition and Food Science, 26(2), 219-229. https://doi.org/10.3746%2Fpnf.2021.26.2.219
Flyman, M. V., & Afolayan, A. J. (2007). Proximate and mineral composition of the leaves of Momordica balsamina L.: An under-utilized wild vegetable in Botswana. International Journal of Food Sciences and Nutrition, 58(6), 419-423. https://doi.org/10.1080/09637480701253417
Fraizer, R. A. (2009). Food chemistry. Wiley-Blackwell.
Haimed, Y. A. S., Samaddar, S., Abdalla, A. M., & Jha, D. K. (2019). An investigation on antidiabetic activity of phytochemical(s) isolated from Momordica dioica in type I diabetes mellitus. International Journal of Research and Analytical Reviews, 6, 786-798.
Harbers, L. H. (1988). Ash analysis. In S. S. Nielsen (ed.), Food Analysis (2nd ed.). Aspen.
Hassan, L. G., & Umar, K. J. (2006). Nutritional value of balsam apple (Momordica balsamina L.) leaves. Pakistan Journal of Nutrition, 5(6), 522-529. https://doi.org/10.3923/pjn.2006.522.529
Hassan, M. M., Uddin, S., Bhowmik, A., Ashraf, A., Islam, M. M., & Rokeya, B. (2022). Phytochemical screening and antidiabetic effects of fruit rind of Momordica dioica roxb. On streptozocin induced type 2 diabetic rats. Heliyon, 8(1), e08771. https://doi.org/10.1016/j.heliyon.2022.e08771
Hawkes, C. (2006). Uneven dietary development: Linking the policies and processes of globalization with the nutrition transition, obesity and diet-related chronic diseases. Global Health, 2, 4. https://doi.org/10.1186/1744-8603-2-4
Heinrich, M., Mah, J., & Amirkia, V. (2021). Alkaloids used as medicines: Structural phytochemistry meets biodiversity-an update and forward look. Molecules, 26(7), 1836. https://doi.org/10.3390/molecules26071836
Horax, R., Hettiarachchy, N., Kannan, A., & Chen, P. (2010). Proximate composition and amino acid and mineral contents of Momordica charantia L. pericarp and seeds at different maturity stages. Food Chemistry, 122(4), 1111-1115. https://doi.org/10.1016/j.foodchem.2010.03.093
Idris, O. A., Wintola, O. A., & Afolayan, A. J. (2019). Comparison of the proximate composition, vitamins (ascorbic acid, -tocopherol and retinol), anti-nutrients (phytate and oxalate) and the GC-MS analysis of the essential oil of the root and leaf of Rumex crispus L. Plants, 8, 51.
Iqbal, E., Salim, K. A., & Lim, L. B. L. (2015). Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University-Science, 27(3), 224-232. https://doi.org/10.1016/j.jksus.2015.02.003
Jansen van Rensburg, W. S., van Averbeke, W., Slabbert, R., Faber, M., van Jaarsveld, P., van Heerden, I., Wendhold, F., & Oelofse, A. (2003). African leafy vegetables in South Africa. Water SA, 33(3), 317-326. https://doi.org/10.4314/wsa.v33i3.49110
Jéquier, E. (1994). Carbohydrates as a source of energy. The American Journal of Clinical Nutrition, 59(3 Suppl.), 682s-685s. https://doi.org/10.1093/ajcn/59.3.682s
Johns, R., Arun, O. S., & Samuel, S. (2022). Bio activity study of Momordica dioica Rox.ex willd. Journal of Pharmacognosy and Phytochemistry, 11, 89-95.
Joslyn, M. A. (1970). Moisture content and total solids. In M. A. Joslyn (ed.), Methods in food analysis (2nd ed.). Academic Press.
Karaye, I. U., Aliero, A. A., Muhammad, S., & Bilbis, L. S. (2013). Evaluation of nutrient and anti-nutrient contents of selected Nigerian cucurbits seeds. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 4(1), 137-142.
Kebede, T., Gadisa, E., & Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PLOS One, 16(3), e0249253. https://doi.org/10.1371/journal.pone.0249253
Khan, A., Khan, S. A., Khan, S., Zia-ul-islam, S., Baber, N. K., & Khan, M. (2018). Nutritional complications and its effects on human health. Journal of Food Science and Nutrition, 1(1), 17-20. https://doi.org/10.35841/food-science.3.5.17-20
Kopylov, A. T., Malsagova, K. A., Stepanov, A. A., & Kaysheva, A. L. (2021). Diversity of plant sterols metabolism: The impact on human health, sport, and accumulation of contaminating sterols. Nutrients, 13(5), 1623. https://doi.org/10.3390/nu13051623
Kumar, G. M. P., Chikkappaiah, L., & Nagayya, S. (2016). Nutritional analysis of edible wild plants used by Hakki Pikki Tribes of Hassan District, Karnataka, India. International Journal of Pharmacy and Pharmaceutical Sciences, 8, 390-393.
Kumar, N., & Khurana, S. M. P. (2016). Nutritive and antidiabetic benefits of Momordica charantia L. (bitter gourd). Indo American Journal of Pharmaceutical Sciences, 3(12), 1512-1520.
Liu, A. G., Ford, N. A., Hu, F. B., Zelman, K. M., Mozaffarian, D., & Kris-Etherton, P. M. (2017). A healthy approach to dietary fats: Understanding the science and taking action to reduce consumer confusion. Nutrition Journal, 16(1), 53. https://doi.org/10.1186/s12937-017-0271-4
Lunn, J., & Buttriss, J. L. (2007). Carbohydrates and dietary fibre. Nutrition Bulletin, 32(1), 21-64. https://doi.org/10.1111/j.1467-3010.2007.00616.x
Mabasa, X. E., Mathomu, L. M., Madala, N. E., Musie, E. M., & Sigidi, M. T. (2021). Molecular spectroscopic (FTIR and UV-Vis) and hyphenated chromatographic (UHPLC-qTOF-MS) analysis and in vitro bioactivities of the Momordica balsamina leaf extract. Biochemistry Research International, 2021, 2854217. https://doi.org/10.1155/2021/2854217
Madala, N. E., Piaster, L., Dubery, I., & Steenkamp, P. (2016). Distribution patterns of flavonoids from three Momordica species by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry: A metabolomic profiling approach. Brazilian Journal of Pharmacognosy, 26(4), 507-513. https://doi.org/10.1016/j.bjp.2016.03.009
Malik, T., Madan, V. K., & Dhanda, T. (2020). Assessment of medicinal plants through proximate and micronutrients analysis. Journal of Plant Development Sciences, 12, 223-230.
Mujeeb, F., Bajpai, P., & Pathak, N. (2014). Phytochemical evaluation, antimicrobial activity, and determination of bioactive components from leaves of Aegle marmelos. BioMed Research International, 2014, 497606. https://doi.org/10.1155/2014/497606
Nagarani, G., Abirami, A., & Siddhuraju, A. (2014). Food prospects and nutraceutical attributes of Momordica Species: A potential tropical bioresources – A Review. Food Science and Human Wellness, 3(3-4), 117-126. https://doi.org/10.1016/j.fshw.2014.07.001
Okoroh, P. N., Duru, M. C. K., Onuoha, S. C., & Amadi, B. A. (2019). Proximate composition, phytochemical and mineral analysis of the fruits of Ficus Capensis. International Journal of Innovative Research and Development, 8(9), 83-88. https://doi.org/10.24940/ijird/2019/v8/i9/SEP19021
Omokhua-Uyi, A. G., & Van Staden, J. (2020). Phytomedicinal relevance of South African Cucurbitaceae species and their safety assessment: A review. Journal of Ethnopharmacology, 259, 112967. https://doi.org/10.1016/j.jep.2020.112967
Osinubi, A. D., Animashaun, L. O., Akinsipo, O. B., Badeji, A. A., Muobike, M. C., Anselm, O. H., & Ogundiran, A. A. (2023). Determination of the most efficient solvent for the extraction of phytochemicals of Securidaca longipedunculata (Violet tree) root bark. Journal of Pure and Applied Sciences, 2(1), 59-63.
Osman, M. H., Farrag, E., Selim, M., Osman, M. S., Hasanine, A., & Selim, A. (2017). Cardiac glycosides use and the risk and mortality of cancer; systematic review and meta-analysis of observational studies. PloS One, 12(6), e0178611. https://doi.org/10.1371/journal.pone.0178611
Osuagwu, A. N., & Edeoga, H. O. (2014). Nutritional properties of the leaf, seed and pericarp of the fruit of four Cucurbitaceae species from South-East Nigeria. IOSR Journal of Agriculture and Veterinary Science, 7(9), 41-44. https://doi.org/10.9790/2380-07944144
Oyelere, S. F., Ajayi, O. H., Ayoade, T. E., Pereira, G. B. S., Owoyemi, B. C. D., Ilesanmi, A. O., & Akinyemi, O. A. (2022). A detailed review on the phytochemical profiles and anti-diabetic mechanisms of Momordica charantia. Heliyon, 8(4), e09253. https://doi.org/10.1016/j.heliyon.2022.e09253
Park, Y. W., & Bell, L. N. (2004). Determination of moisture and ash contents of foods (2nd ed.). Marcel Dekker, Inc.
Patel, S., Patel, T., Parmar, K., Patel, B., & Patel, P. (2011). Evaluation of antioxidant activity, phenol and flavonoid contents of Momordica charantia linn. fruit. Advance Research in Pharmaceutical and Biologicals, 1, 120-129.
Pinipay, F., Rokkam, R., Bollavarapu, A., Rapaka, G., & Tamanam, R. R. (2022). Phytochemical screening of Ficus religiosa Seeds and evaluation of its antioxidant potential. Journal of Food Chemistry and Nanotechnology, 8(3), 127-137.
Ramalhete, C., Gonçalves, B. M. F., Barbosa, F., Duarte, N., & Ferreira, M. U. (2022). Momordica balsamina: Phytochemistry and pharmacological potential of a gifted species. Phytochemistry Reviews, 21(2), 617-646. https://doi.org/10.1007/s11101-022-09802-7
Rasmi, D. A. C., Zulkifli, L., & Ahadia, N. (2023). Antimicrobial activity test of bitter melon (Momordica charantia L.) plant extract against Staphylococcus epidermidis, Escherichia coli bacteria and Candida albicans. Jurnal Penelitian Pendidikan IPA, 9, 454-458.
Rathnayake, A. M. G. N., Abeysinghe, D. C., Dharmadasa, R. M., Prathapasinghe, G. A., & Jayasooriya, L. J. A. P. A. (2018). Phytochemical, physiochemical and mineral contents of domesticated and non-domesticated populations of Momordica charantia L. seeds harvested at two maturity stages. World Journal of Agricultural Research, 6(3), 77-81.
Redfern, J., Kinninmonth, M., Burdass, D., and Verran, J. (2014). Using ndian ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. Journal of Microbiology and Biology Education, 15(1), 45-46. https://doi.org/10.1128%2Fjmbe.v15i1.656
Saeed, M. K., Shahzadi, I., Ahmad, I., Ahmad, R., Shahzad, K., Ashraf, M., & Viqar-un-Nisa. (2010). Nutritional analysis and antioxidant activity of bitter gourd (Momordica charantia) From Pakistan. Pharmacology Online, 1, 252-260.
Salvi, J. (2015). Nutritional composition of Momordica dioica Fruits: As a wild vegetable. Journal of Food and Pharmaceutical Sciences, 3(2), 18-22. https://doi.org/10.14499/jfps
Samanta, A., Das, G., & Das, S. K. (2011). Roles of flavonoids in plants. International Journal of Pharmacy and Pharmaceutical Sciences, 6, 12-35.
Sawai, S., & Saito, K. (2011). Triterpenoid biosynthesis and engineering in plants. Frontiers in Plant Science, 2, 25. https://doi.org/10.3389%2Ffpls.2011.00025
Seniya, C., Trivedia, S. S., & Verma, S. K. (2011). Antibacterial efficacy and phytochemical analysis of organic solvent extracts of Calotropis gigantea. Journal of Chemical and Pharmaceutical Research, 3(6), 330-336.
Shi, J., Arunasalam, K., Yeung, D., Kakuda, Y., Mittal, G., & Jiang, Y. (2004). Saponins from edible legumes: chemistry, processing, and health benefits. Journal of Medicinal Food, 7(1), 67-78. https://doi.org/10.1089/109662004322984734
Sibiya, N. P., Kayitesi, E., & Moteetee, A. N. (2021). Proximate analyses and amino acid composition of selected wild indigenous fruits of Southern Africa. Plants, 10(4), 721. https://doi.org/10.3390/plants10040721
Slavin, J., & Carlson, J. (1994). Carbohydrates. Advances in Nutrition, 5(6), 760-761. https://doi.org/10.3945/an.114.006163
Sudhakararao, G., Priyadarsini, K. A., Kiran, G., Karunakar, P., & Chegu, K. (2019). Physiological role of proteins and their functions in human body. International Journal of Pharma Research and Health Sciences, 7(1), 2874-2878. https://doi.org/10.21276/ijprhs.2019.01.02
Sulaiman, A., & Yakubu, M. (2018). Chemical composition of Momordica charantia leaves. International Journal of Advances in Science Engineering and Technology, 6(2), 2321-8991.
Tapsell, L. C., Neale, E. P., Satija, A., & Hu, F. B. (2016). Foods, nutrients, and dietary patterns: Interconnections and implications for dietary guidelines. Advances in Nutrition, 7(3), 445-454. https://doi.org/10.3945/an.115.011718
Thakur, G. S., Bag, M., Sanodiya, B. S., Bhadauriya, P., Debnath, M., Prasad, G. B. K. S., & Bisen, P. S. (2009). Momordica balsamina: A medicinal and nutraceutical plant for health care management. Current Pharmaceutical Biotechnology, 10(7), 667-682. https://doi.org/10.2174/138920109789542066
Uchegbu, R. I., Bako, S. S., Amanze, K. O., & Igara, C. E. (2017). An evaluation of the chemical composition and antibacterial activities of Mormodica balsamina seeds extract. Recent Advances in Petrochemical Science, 1(4), 555569. https://doi.org/10.19080/RAPSCI.2017.01.555569
Ülger, T. G., Ayşe, N. S., Onur, C., & Çakıroğlu, F. P. (2018). Role of vegetables in human nutrition and disease prevention. Intechopen.
Upadhyay, A., Agrahari, P., & Singh, D. K. (2015). A review on salient pharmacological features of Momordica charantia. International Journal of Pharmacology, 11(5), 405-413. https://doi.org/10.3923/ijp.2015.405.413
Ushie, O. A., Longbap, B. D., Iyen, S. I., Ugwuja, D. I., Azuaga, T. I., & Fidelis, H. (2022). Phytochemical screening and antioxidants investigations of ethyl acetate and acetone leaf extracts of Thaumatococcus danielli. Research Journal of Pharmaceutical, Chemical and Biological Sciences, 3(1), 001-006. https://doi.org/10.5281/zenodo.6419653
Ushie, O. A., Onen, A. I., Ugbogu, O. C., Neji, P. A., & Olumide, V. B. (2016). Phytochemical screening and antimicrobial activities of leaf extracts of Swietenia macrophylla. ChemSearch Journal, 7(2), 64-69.
World Health Organization (WHO). (2019). Malnutrition is a world health crisis. WHO. Retrieved from https://www.who.int/news/item/26-09-2019-malnutrition-is-a-world-health-crisis
Yadav, P., Kumar, A., & Vihan, V. S. (2010). Phytochemical analysis of some indigenous plants potent against endoparasite. Journal of Advanced Laboratory Research in Biology, 1(1), 56-59.
Yerima, B. I., & Umar, A. (2019). Evaluation of the chemical and oil composition of balsam apple (Momordica balsamina) seed. Asian Journal of Chemical Sciences, 6, 1-13. https://doi.org/10.9734/ajocs/2019/v6i318998
Zahan, S., Mowla, T. E., Uddin, S. M. N., Hossain, M. K., Mannan, A. B., Rahman, M., Chen, U., Mazumder, T., Uddin, A. H. M. M., Arefin, S., & Hussain, M. S. (2020). Evaluation of phytochemical and pharmacological properties of seeds of Momordica charantia. Avicenna Journal of Phytomedicine, 10(5), 448-459.
